Comparison of Platelet Count by Automated and Manual Methods in Thrombocytopenia Patients

Abstract

Objective: This study aimed to investigate platelet count variations in adult thrombocytopenia patients by comparing automated platelet counts with manual counting methods.

Methodology: A comparative cross-sectional study was conducted at the Hematology department of King Edward Medical University, Lahore from January 2022 to June 2022, involving 60 patients with thrombocytopenia. Complete blood counts were performed using an Automated Hematology Analyzer, and peripheral smears were prepared and manually examined by two experts to verify platelet counts.

Results: The study included 31 females and 29 males, with a mean age of 43.7 years. The mean platelet count obtained from automated analyzers was 58 ± 28×10^9/L, while the manually verified platelet count on peripheral smears was 117 ± 13×10^9/L, with a significant p-value of <0.001. Pseudo-thrombocytopenia was observed in 52% of patients, primarily due to platelet clumps (42%) and giant platelets (39%).

Conclusion: The study underscores the importance of manual verification of platelet counts in thrombocytopenic patients, as automated counts tended to underestimate platelet levels. Peripheral smears remain the gold standard for accurate platelet counting, helping prevent unnecessary investigations and ensuring appropriate patient care

Key words: Diagnostic accuracy, automated haematology analyser, sysmexXN-1000, malaria, microscopy.