Effect of Platelet Functions Studies during 3 Days of Storage of Single Donor Platelets Obtained From Platelet-Apheresis

Abstract

Purpose: Platelet apheresis is a method to extract platelet from the whole blood in a donor. These helps in the blood coagulation but their storage condition could hinder their proper function after 72hour of storage.

Methodology:

This study was conducted at department of Hematology Children Hospital Lahore after obtaining informed consent from the donor by a trained staff member. Samples were chosen irrespective of gender and with the exact age of the cases. All the sample who have <250,000/ µL platelet count were rejected. 92 sample were taken based on the WHO sample size estimated.

Platelet concentration, aggregation was evaluated using different concentrations of inducing agonists: ADP (7.0 µg/mL). For each test, 400 µL of platelet rich plasma was used, each one in a different cuvette after waiting for spontaneous aggregation. The aggregation curve was observed after five minutes of stimulation by inducing agonists¹⁰. Chrono-log aggregometer was used for aggregation studies at 0day and 3^rd day. SPSS was used for data analysis.

Result:

The average age of donors was 29.38(yr). More then half were female donor. Mean platelet count was 270300 µL. When aggregation of the platelet count was evaluated, it was found that 24(26.1%) have aggravated platelet at day 0. While on day 3, platelet aggregation was fond in 11(12%) of cases The percentage decrease in aggregation of platelet was 49(53.3%).

Conclusion:

There is reduction in platelet aggregation in the patients when they were evaluated at day0 and day 3.